AFFINILUTE™ MIP - Nitroimidazole

Class-selective SPE sorbent for extraction of nitroimidazoles from food samples (e.g. milk and egg samples)

Nitroimidazoles are antibacterial and anticoccidial drugs used for treatment of cattle, poultry, pigs and farmed fish. These compounds and their metabolites are suspected carcinogens and mutagens. Consequently, several nitroimidazoles are classified in the European Union (EU) list of pharmacologically active substances for which no maximum residue limit can be fixed, as such, their use is forbidden in treating food-producing animals. Some compounds in the class have never been authorized as a veterinary drug and are also banned for use in animals. Monitoring these substance residues at trace levels is therefore regulated by law.

Features and Applications
AFFINILUTE MIP - Nitroimidazoles is a simple, fast, selective and sensitive SPE product for selective extraction of nitroimidazole residues from complex matrices. The SPE phase has been developed for multi-residue extraction of nitroimidazoles and has been validated for Dimetridazole (DMZ), ipronidazole (IPZ), metronidazole (MNZ), and ronidazole (RNZ) and their hydroxylated metabolites (DMZOH and MNZOH). Using AFFINILUTE MIP -Nitroimidazoles were extracted from milk and egg samples (fresh eggs and egg powder) with high and reproducible recoveries with low RSD.

A data sheet with general guidelines for this SPE is available. Specific applications for extraction of nitroimidazoles from milk and egg samples with LC-MS/MS detection is available.