AFFINILUTE™ MIP - TSNAs

Class selective SPE sorbent for multi-residue extraction of 4 different Tobacco specific Nitrosamines: NNK, NNN, NAB and NAT

Tobacco-specific nitrosamines (TSNAs) are a class of known carcinogens that are found only in tobacco products. The TSNAs are created during fermentation, curing and burning of the tobacco leaf.

People are exposed to TSNAs by using tobacco and by exposure to second-hand smoke. TSNAs show the highest concentration of any group of strong carcinogens in cigarette smoke. NNN, NNK, NAB and NAT are the most common TSNAs in tobacco products with NNN and NNK being the most carcinogenic compounds. Exposure to TSNAs from tobacco products can be confirmed by their detection in a variety of biological samples e.g. saliva and urine. TSNAs can also be measured in tobacco extracts.

Abbreviations:
NNN = N'-nitrosonornicotine, NNK=(4-methylnitrosamino)-1-(3-pyridyl)-1-butanone, NAT=N'-nitrosoanatabine, NAB=N'-nitrosoanabasine

Features and Applications
With this sorbent the 4 different tobacco specific nitrosamines can be selectively extracted from biological samples with high recoveries. Compared with current analytical methods for accurate TSNA detection, which are elaborate and time-consuming, AFFINILUTE MIP - TSNAs provides reliable and accurate extraction and reduces the sample handling time significantly. It allows extremely low limits of quantification and has been shown to be very reproducible.